Journal:

Article Title: Germ Line Transmission of the Cdk4 R24C Mutation Facilitates Tumorigenesis and Escape from Cellular Senescence

doi: 10.1128/MCB.22.2.644-656.2002

Figure Lengend Snippet: Expression of proteins p16Ink4a, p19ARF, p21Cip1, and p53 in Cdk4+/+ and Cdk4R24C/R24C early- and late-passage MEF cultures. Protein extracts from Cdk4+/+ and Cdk4R24C/R24C (n = 4) (R24C/R24C #1 to #4) early- and late-passage MEF cultures (passages 1, 5, 10, 15, 20, and 25) were resolved by SDS-12% PAGE and subjected to Western blot analysis using antibodies to p16Ink4a, p19ARF, p21Cip1, and p53. Not enough Cdk4+/+ cells could be harvested at passage 25 for the generation of protein extracts. The migration of proteins is identified by arrows.

Article Snippet: Antibodies used include rabbit polyclonal antibodies from Santa Cruz Biotechnology: anti-Cdk4 (C-22), anti-p16 (M-156), anti-p21Cip1 (C-19), anti-p53 (FL-393), anti-p130 (C-20), anti-p107 (C-18), and anti-pRb (G3-245; 14001A) antibodies were from BD Transduction Laboratories and anti-p19ARF (NB-200-106) antibody was from Novus Biologicals.

Techniques: Expressing, Western Blot, Migration

Journal: BioMed Research International

Article Title: Role of Estrogens in the Size of Neuronal Somata of Paravaginal Ganglia in Ovariectomized Rabbits

doi: 10.1155/2017/2089645

Figure Lengend Snippet: Primary and secondary antibodies and normal sera used in the present study.

Article Snippet: Rabbit polyclonal IgG anti-aromatase , 1 : 500 , NB-200-1596 , Novus Biologicals.

Techniques:

Journal: BioMed Research International

Article Title: Role of Estrogens in the Size of Neuronal Somata of Paravaginal Ganglia in Ovariectomized Rabbits

doi: 10.1155/2017/2089645

Figure Lengend Snippet: Serum concentrations of total estradiol (E2, (a)) and testosterone (T, (b)) vary between the C, OVX, and OVX + EB groups. (c) The ratio of E2 to T (as logarithm) was calculated to estimate the extent of extragonadal aromatization. Data are the mean ± SEM ( n = 6 per group). (d) Aromatase expression in the ovary (O) and vagina (V) for control rabbits; Ponceau's Red staining was used to corroborate equal amounts of protein were loaded. Paravaginal neurons from the C (e), OVX (f), and OVX + EB (g) groups express aromatase as showed by immunohistochemistry. (h) Percentages of aromatase-ir neurons are means ± SEM ( n = 6 per group). One-way ANOVA followed by Newman–Keuls post hoc tests were carried out to determine significant differences between groups. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 (compared to the C group); & P < 0.001 (compared to the OVX group). −n, negative neurons; +n, positive neurons; +SGC, positive satellite glial cell. Bar, 20 µ m.

Article Snippet: Rabbit polyclonal IgG anti-aromatase , 1 : 500 , NB-200-1596 , Novus Biologicals.

Techniques: Expressing, Staining, Immunohistochemistry